Ruminant B-Lymphocyte Yellow Fluorescent Protein Aggregation Bioassay for Elk Chronic Wasting Disease

Period of Performance: 08/12/2004 - 03/12/2005

$100K

Phase 1 STTR

Recipient Firm

Nomadics, Inc.
1024 S. Innovation Way
Stillwater, OK 74074
Firm POC
Principal Investigator

Research Institution

Oklahoma State University
201 Advanced Technology Research Center
Stillwater, OK 74078
Institution POC

Abstract

The goal of the proposed research is to develop a cell culture model for elk chronic wasting disease (CWD) prion propagation that can be used as a bioassay for detecting CWD. Our goal will be accomplished by bioengineering a bovine B-lymphocyte cell (B-cell) line to surface express elk PrPc fused to yellow fluorescent protein (YFP). Interaction of these B-cells with infectious, mis-folded, protease resistant CWD prion (PrPcwd) will induce conversion of the dispersed YFP-PrPc to aggregated mis-folded YFP-PrPcwd that will be detected by confocal microscopy as aggregated YFP-PrPcwd on the B-cell surface. Once Phase I proof-of-concept has been demonstrated by elk PrPcwd conversion of YFP-PrPc on the B-cell line to YFP-PrPcwd, a stably transfected cell line will be developed and validated as a sensitive, specific, and reproducible bioassay for detecting PrPcwd infected elk and deer tissues and body fluids during Phase II.