High Resolution Imaging Ellipsometer for Labeless, Multi-Channel Immunosensing

Period of Performance: 05/15/1998 - 11/15/1998

$100K

Phase 1 SBIR

Recipient Firm

Physical Optics Corp.
1845 West 205th Street Array
Torrance, CA 90501
Principal Investigator

Abstract

We propose to develop a real-time imaging optical arrangement monitoring a sample solution for a large number of analytes in parallel. Using a photo-induced immobilization technique, a multitude of different antibodies is immobilized on the surface of a glass carrier, each type located at a specific position on the chip. When sample fluid containing the analytes lows over the sensor surface, the top plane of the optical carrier chip is interrogated from below by an imaging ellipsometer to provide a spatially resolved measurement of the sample surface in approximately one second. This allows precise monitoring of the film thickness variations due to a binding event with monolayer resolution. The use of several spectral bands for performing the measurement adds another parameter in addition to film thickness determination to enhance the sensitivity. The imaging will be performed with about 10 micrometer lateral resolution, leading to highly resolved fast information about the binding status of a vast number of different antibodies without the need for labeling reagents. The optical setup can also be used with minor modifications to monitor DNA binding events. This system will be of high commercial interest for chemical and biological screening and for environmental monitoring applications.