Detection of Arthropod-Borne Pathogens by Rapid Panel Assays in Mosquitoes

Period of Performance: 04/01/1998 - 04/01/2000

$746K

Phase 2 SBIR

Recipient Firm

Navix, Inc.
542 Flynn Rd
Camarillo, CA 93012
Principal Investigator

Research Topics

Abstract

The overall goal of Phase II of this project is to develop a rapid, one step, rugged immuno-chromatographic dip-stick assay for the identification of malaria-infected mosquitoes. This assay is based on the existing know-how at Navix by which sensitive test-strip based single analyte or panel assays provide results in less than 15 minutes and the tests are stable at ambient storage conditions. The results of Phase I have demonstrated that the antibody reagents provided by the contracting agency against various malarial sporozoite antigens can be used effectively to generate highly sensitive and robust assays economically. Phase II will involve: (1) Production and testing of a consistent product. Tests will be produced in quantities where significant data can be generated and evaluated. As the project matures, at various time lines, updated formats will be provided to ensure improvement in performance or results. (2) The tests will be compared to the available "gold-standard" assays. (3) Development of panel assays, where single assays capable of detecting several different species/polymorphs of human malaria sporozoites will be developed. (4) Development of panel assays for other targets such as for the detection of Dengue virus types 1 to 4, flaviviruses, alphaviruses and phleboviruses. BENEFITS: Economic and timely identification of arthropod-borne disease threat will benefit both the Department of Defense (DoD) and the various civilian health agencies throughout the world. Since the present technology furnishes an assay system that can be stared ambiently, it can be transported and used without any loss in performance for over 12 months. The proposed assay system is simple to use and interpret, and developed to be effective in adverse environment.