Nanocrystal FRET Probes for Multi-Wavelength Analysis of Protein Dynamics

Period of Performance: 12/14/2006 - 08/14/2007

$98.9K

Phase 1 SBIR

Recipient Firm

Luna Innovations, Inc.
301 1st St Suite 200
Roanoke, VA 24011
Principal Investigator

Abstract

Luna Innovations will demonstrate the feasibility of using a novel set of nanoparticle fluorescence resonant energy transfer (FRET) probes to monitor protein dynamics via real-time single-molecule spectroscopy. The proximity-dependent FRET-like interaction between nanoparticles would translate into time-dependent spectroscopic monitoring of changes in a protein molecule s shape. The nanoparticle emitters will meet the following probe constraints: fluorescence FWHM less than 10 nm, probe diameter less than 3 nm, photoluminescence quantum yield (PLQY) greater than 90%, suspension in water, conjugation with antibodies. Strategies for optimizing the synthesis of these nanoparticles specifically for this application will be reviewed.