Novel Membrane System to Automatically Deglycerolize Thawed Frozen Human Blood

Period of Performance: 03/15/1995 - 08/14/1997

$694K

Phase 2 SBIR

Recipient Firm

Advanced Haemotechnologies
2828 N. Crescent Ridge Drive
The Woodlands, TX 77381
Principal Investigator

Research Topics

Abstract

Improved methods for frozen storage of glycerolized red blood cells (RBCs) are needed both to meet military and civilian needs in periods of high demand. Existing deglycerolization systems are labor intensive and considered "open" systems resulting in a maximum storage of 24 hours following washing. The washing of blood cells with flat sheet membrane filters has been proven effective by the Advanced Haemotechnologies PlateletPlus TM Autotransfusion System. AHT completed Phase I development of a novel membrane device which is capable of washing a unit of frozen-thawed RBCs in less than 20 minutes, with a residual glycerol level of less than 1%, and a final hematocrit of approximately 40%. Phase II development will include: optimization of the disposable to yield higher hematrocrits, low glycerol residuals and low supernatant hemoglobin levels; design and prototyping a console with evaluation by the NBRL of performance; and preliminary evaluation of a modified filter for washing frozen/thawed platelets. Sucessful completion of the Phase II development will lead to a closed, sterile washing system for thawed blood and should allow for extended storage thawed RBC products.