Large Field of View Microscope for Rapid, High-Resolution Imaging

Period of Performance: 07/31/2017 - 01/30/2018

$98.2K

Phase 1 SBIR

Recipient Firm

Nikira Labs, Inc.
1931 Old Middlefield Way Suite 208
Mountain View, CA 94043
Firm POC, Principal Investigator

Abstract

In this SBIR effort, Nikira Labs Inc. proposes to develop a high-resolution (< 1 micron) microscope that can rapidly (< 30 seconds) image a large field of view (> 1 cm2). The technology will provide both bright-field and fluorescence imaging and be capable of retrofitting into a wide variety of existing microscopes at a low retail price. This work will complement the microscopy efforts that are ongoing at the National Institute of Standard and Technology (NIST). In Phase I, we will demonstrate technical feasibility by fabricating two optical microscopes that utilize Fourier Ptychographic Imaging (FPI) via highly controlled, inhomogeneous illumination sources. The first imaging system will target high-performance applications, whereas the second system will be more cost-effective for educational applications. In both cases, the illumination source will be improved to increase the imaging speed, and the camera will be optimized for lower noise and cost. Image analysis and reconstruction software will be developed in a platform-portable language to allow for retrofitting on existing systems. The Phase I prototypes will be characterized to determine their resolution, field Large Field of View Microscope for Rapid, High-Resolution Imaging Nikira Labs Inc. of view (FOV), imaging speed, and depth-of-focus. Finally, Phase II instruments will be designed to include fluorescence and phase imaging.