A rapid, culture-free whole genome approach for characterizing complex metagenomics samples

Period of Performance: 09/01/2017 - 08/31/2018

$938K

Phase 2 SBIR

Recipient Firm

Dovetail Genomics, LLC
Santa Cruz, CA 95060
Principal Investigator

Abstract

Project Summary / Abstract Dovetail Genomics has adapted our in vitro proxity- ligation method for fecal metagenomics applications. We are developing a powerful and efficient approach to de novo metagenomics assembly that will allow the research and biomedical communities to move beyond single locus molecule counting or statistical inference. Successful development of this approach will provide a single, integrated workflow for accurate assembly of all major constituents of complex metagenomics communities. This tool will enable a comprehensive understanding of the ways our gut microbiome influences human health and disease. In this project, Dovetail Genomics will develop the modifications to the laboratory procedures necessary to efficiently capture and represent the diversity of microbes present in a human fecal sample. We will also modify and develop a new computational approach to metagenomics assembly that exploits the rich datatype that we generate. This computational approach will achieve highly contiguous scaffolding and strain deconvolution. We envision a robust, fool- proof laboratory protocol and software product that allows a clinician to generate a comprehensive view of the dynamic human gut microbial environment from a small fecal sample in a manner of days.