Tetrodotoxin Immunoassays

Period of Performance: 01/01/1988 - 12/31/1988

$509K

Phase 2 SBIR

Recipient Firm

Hawaii Biotech, Inc.
HONOLULU, HI 96817
Principal Investigator

Abstract

TETRODOTOXIN IS A HIGHLY POTENT LOW MOLECULAR WEIGHT NEUROTOXIN FOUND IN SOME SPECIES OF PUFFER FISH AND A FEW OTHER WIDELY DIVERGENT ORGANISMS. CURRENTLY, TETRODOTOXIN IS MEASURED BY MOUSE BIOASSAY OR WITH SOPHISTICATED CHROMATOGRAPHIC METHODS. A SIMPLE, SPECIFIC, EASY TO USE METHOD, SUCH AS AN IMMUNOASSAY FOR TETRODOTOXIN IS NEEDED. TO DATE, NO IMMUNOASSAY FOR TETRODOTOXIN EXISTS, PRESUMABLY BECAUSE OF DIFFICULTIES ASSOCIATED WITH THE CHEMISTRY OF TETRODOTOXIN. WE HAVE SYNTHESIZED TWO DERIVATIVE OF TETRODOTOXIN WHICH MAY NOW BE USED AS HAPTENS FOR THE PRODUCTION OF ANTITETRODOTOXIN ANTIBODIES. PHASE II FOCUSES ON THE PROBLEM OF GENERATING A RELIABLE IMMUNOASSAY FOR TETRODOTOXIN. SUCH AN ASSAY COULD FILL THE NEED TO DETECT TETRODOTOXIN IN A WIDE VARIETY OF BIOLOGICAL MATRICES.