Portable Cell Maintenance System

Period of Performance: 11/22/2005 - 10/21/2006

$787K

Phase 2 STTR

Recipient Firm

Agave Biosystems, Inc.
P.O. Box 100
Ithaca, NY 14850
Principal Investigator
Firm POC

Research Institution

Cornell University
426 Phillips Hall
Ithaca, NY 14853
Institution POC

Abstract

Cultured cell-based biosensors offer insight into the physiological action of the agent of interest, which is an advantage over other types of sensors. The development of cell-based biosensors that are field-portable would increase their utility in toxicology and environmental monitoring. One of the most significant issues hampering the development of field portable cell-based biosensors is the maintenance of optimal cultured cell environment under field conditions. In the Phase I work, Agave BioSystems and Cornell University successfully demonstrated proof of concept for a cell maintenance system (CMS) with integrated pH, osmolarity, CO2 and temperature sensors to support multiple vertebrate cells and cell types. In the Phase I CMS instrument demonstration, endothelial cells, fibroblast cells, and neuronal networks were supported for at least 3 days. In the proposed Phase II effort, a compact, portable and rugged prototype cell maintenance system will be developed and constructed with integrated monitoring and environmental control. The system will be designed to maintain a variety of cell types for more than 30 days.