Metabolic Reconstruction of the Bacillus Cereus Genome

Period of Performance: 05/22/2001 - 08/30/2002

$500K

Phase 2 STTR

Recipient Firm

Integrated Genomics, Inc.
2201 West Campbell Drive,
Chicago, IL 60612
Principal Investigator
Firm POC

Research Institution

University of Illinois, Chicago
809 S Marshfield RM 608
Chicago, IL 60612
Institution POC

Abstract

Comparative genomics is an efficient approach to characterize a range of pathogenic properties, from sporulation and pathogenicity factors to the evolution of multi-drug resistance caused by high affinity transport and ion efflux pump systems. IG's WIT-ProTM and "Informatics Workbench" are an optimal system for such studies. In Phase I, we completed the gapped sequence of B. cereus with 4.1x redundancy. 6300 ORFs were predicted, compared with IG's database, and subjected to automate functional assignment in WIT-ProTM, which also connects the protein products they encode to potential metabolic pathways. In Phase II, we will generate a polished sequence of B. cereus and perform comparative genomic analyses with B. anthraces and B. thuringiensis. We will increase the coverage of B. cereus to 8 fold, and perform primer walking. We will integrate the final sequence into our WIT ProTM environment and apply various algorithms to verify the automated functional predictions. We will also generate a gapped sequence of B. thuringiensis. We will compare these two sequences with the DNA sequence of B. anthraces generated by TIGR, using our specialized set of software tools to reveal the specific features of B. anthraces and to generate a functional overview of this group of organisms. Integrating these organisms into our genome database, MicrobAceTM, consisting of 19 organisms sequenced at IG, 80 complete or nearly complete public genomes, and sequence data from another 200 organisms (~440,000 ORFs), will improve the database quality since members from a very under-represented taxonomic group will be included. In addition, the comparative analysis of B. anthraces should reveal important species and strain differences related to the pathogenicity of this organism.