SBIR Phase I: Vibrational CD Microscopy for Characterizing Supramolecular Bio-Chirality

Period of Performance: 01/01/2010 - 12/31/2010

$125K

Phase 1 SBIR

Recipient Firm

BIOTOOLS, INC.
17546 Bee Line Hwy
Jupiter, FL 33458
Principal Investigator

Abstract

This Small Business Innovation Research (SBIR) Phase I project is focused on the creation of a new revolutionary imaging instrumentation that combines vibrational circular dichroism (VCD) spectroscopy with infrared (IR) spectral microscopy. VCD microscopy represents a new class of spectroscopic imaging diagnostic capable of measuring VCD images with millimeter to sub-millimeter spatial resolution. The recent discovery that long-range structural chirality in protein fibrils is characterized by unusually large and distinctive VCD spectra provides the backdrop for this project. None of the currently available techniques can characterize the fibrillation pathway or the final fibril state with the same ease and detail as VCD. VCD microscopy can be thought of as circular polarization contrast microscopy that is sensitive to long-range chiral order in localized regions of biological samples.