Biosensor for Field Monitoring of Pesticides in Water

Period of Performance: 01/01/2006 - 12/31/2006


Phase 2 SBIR

Recipient Firm

Eltron Research, Inc.
4600 Nautilus Court South
Boulder, CO 80301
Firm POC
Principal Investigator


This SBIR Phase II proposal addresses development of a field-portable, amperometric biosensor for monitoring organophosphorus (OP) and carbamate insecticides in water. The miniaturized biosensor will use a modified screen-printed microelectrode with high sensitivity and lowlimit of detection for OP and carbamate pesticides. This biosensor will be selective for OPs and carbamates. The enzyme used to generate the amperometrically detectable species is selectively inhibited by these insecticides, but not by other common classes of agricultural chemicals, such as triazine herbicides. This device is a single use, disposable monitoring chip, since some of the active pesticides, e.g., the OPs, irreversibly inhibit enzyme activity. Carbamate inhibition is more reversible, but the device, in this case, was still evaluated for a single use mode.