Miniaturized Sample Preparation Module

Period of Performance: 05/11/2000 - 11/11/2000

$70K

Phase 1 SBIR

Recipient Firm

Mesosystems Technology, Inc.
415 N. Quay, Bldg A, Suite 3
Kennewick, WA 99336
Principal Investigator

Abstract

MesoSystems Technology, Inc. (MesoSystems) proposes to design and build an integrated mesofluidic-based biosensor system to sample and detect pathogens from air and soil. The air sampling pathogen detection system will incorporate a MesoSystems miniature rotating arm aerosol collector that concentrates bio-aerosols into water. The resulting fluid samples, containing the collected bio-aerosols, are sent to a combined pathogen bead concentrator and lysis device. The fluid is minimized by passing the sample through an acoustic concentrator, and conveyed to the polymerase chain reaction (PCR) thermal cycler. After DNA amplification, the labeled sample fluoresces and the intensity is correlated with concentration. This miniaturized bio-detector approach serves as an example of the types of biological warfare (BW) detectors now emerging for military use. We propose a compact, lightweight, BW detector system with fluidic interconnections at a scale somewhat larger than microfluidic. Benefits include a product that is rugged for use in battlefield conditions and that is simple to use by soldiers under duress for BW detection.