Validation of a Rapid Progestin-Based Endocrine Disruption Screening Assay

Period of Performance: 01/01/2002 - 12/31/2002

$70K

Phase 1 SBIR

Recipient Firm

Fort Environmental Laboratories, Inc.
515 South Ducan St.
Stillwater, OK 74074
Principal Investigator

Abstract

Fort Environmental Laboratories, Inc.¿s Phase I research project resulted in the development and standardization of an assay that tests substances that might disturb reproductive and developmental processes in animals by interfering with the endocrine system. The primary goal of the proposed research was to validate and commercialize the Xenopus laevis oocyte maturation germinal vesicle breakdown (GVBD) model as a system for the rapid evaluation of endocrine-disrupting chemicals (EDCs) found in the workplace or the environment. Specifically, a 24-hour X. laevis assay modified from the original work of Pickford and Morris (Environmental Health Perspectives 1999;107(4):285-292) and Lui and Patino (Biology of Reproduction 1993;49:980-988) designed to evaluate progestin-active or anti-progestin EDCs in vitro was standardized and evaluated by conducting a preliminary validation study with a series of known EDCs, compounds found to be inactive, and chemicals with unknown activity.